Influence of E-smoking liquids on human periodontal ligament fibroblasts

Introduction:
This text is a summary of a research study examining the impact of various e-smoking liquids on the viability and proliferation of human periodontal ligament fibroblasts. The study compares the effects of different liquids, including nicotine, propylene glycol, and various flavored liquids such as lime, hazelnut, and menthol. The researchers used several assays and staining techniques to assess cell growth and morphology, and analyzed the data using statistical methods.

Key points:

* The study found that the proliferation rates of cells incubated with nicotine or flavored liquids were reduced compared to controls, with the menthol-flavored liquid causing a statistically significant reduction after 96 hours.
* The ATP detection assay showed similar results, with hazelnut, lime, and menthol-flavored liquids causing a statistically significant reduction in ATP detection compared to untreated cells.
* The visual analysis of stained cells confirmed these findings, with untreated cells and those incubated with propylene glycol showing good proliferation, while those incubated with nicotine, hazelnut, or lime-flavored liquids showed a slight reduction, and those incubated with menthol-flavored liquid showed a strong growth inhibition.
* The migration assay also showed that the menthol-flavored liquid caused a highly statistically significant reduction in cell migration compared to control cells and those treated with nicotine.
* The study used a sample size of n = 6 per group and performed statistical analyses using two-tailed one-way ANOVA, with post-hoc tests employing the Bonferroni correction for multiple testing.
* The researchers note that the manufacturing process for e-liquids is not standardized, and that cancer-inducing substances have been detected in some e-liquids.
* The study concludes that menthol additives in e-cigarettes have a harmful effect on human periodontal ligament fibroblasts, and suggests that their use should be avoided.

Main message:
The study highlights the potential harm of e-smoking liquids, particularly those containing menthol additives. The researchers emphasize the need for further studies on the effectiveness of e-cigarettes for smoking cessation and the potential health effects of e-cigarettes. The study suggests that the use of menthol additives in e-cigarettes should be avoided, given their harmful effects on human periodontal ligament fibroblasts. Overall, the study underscores the importance of regulatory oversight and standardization in the manufacturing and use of e-cigarettes and their components.

Willershausen, Ines, Thomas Wolf, Veronika Weyer, Robert Sader, Shahram Ghanaati, and Brita Willershausen. “Influence of E-Smoking Liquids on Human Periodontal Ligament Fibroblasts.” Head & Face Medicine 10, no. 1 (December 2014): 39. https://doi.org/10.1186/1746-160X-10-39.