Summary
Introduction:
This article reports the results of a study examining the effects of electronic cigarette (EC) exposure on influenza a virus (IaV) infection in human precision-cut lung slices (PCLS) and the role of TRaIL (tumor necrosis factor-related apoptosis-inducing ligand) in this process. The study found that EC exposure increased viral load, TRaIL release, and cytotoxicity in IaV-infected PCLS. additionally, blocking TRaIL signaling reduced cell injury and increased intracellular viral load while decreasing viral release into the supernatants. Recombinant TRaIL protein promoted cell injury and the release of viruses from the intracellular compartment into the supernatant.
Key Points:
* The study used human PCLS to examine the effects of EC exposure on IaV infection
* EC exposure increased viral load, TRaIL release, and cytotoxicity in IaV-infected PCLS
* Blocking TRaIL signaling reduced cell injury and increased intracellular viral load while decreasing viral release into the supernatants
* Recombinant TRaIL protein promoted cell injury and the release of viruses from the intracellular compartment into the supernatant
Main Message:
The study demonstrates that EC exposure increases the severity of IaV infection in human PCLS through dysregulation of TRaIL expression. The findings suggest that TRaIL may serve as a mechanism to regulate viral infection during lung exposures to ECs and that maintaining appropriate levels of TRaIL may be important to control IaV infection in EC users. The study also highlights the potential of using human PCLS as a model to study the mechanisms and new therapeutic targets for EVaLI.
Citation
agraval h, Crue T, Schaunaman N, Numata M, Day BJ, Chu hW. Electronic Cigarette Exposure Increases the Severity of Influenza a Virus Infection via TRaIL Dysregulation in human Precision-Cut Lung Slices. International journal of molecular sciences. 2023;24(5). doi:10.3390/ijms24054295
