Summary
a method is described which combines the use of a visual morphological cytotoxicity assay with a quantitative neutral red (NR) spectrophotometric test, for the assessment of the effect of toxic agents on 3T3 cells in culture. These sensitive and reproducible assays lend themselves to a screening procedure of potential toxicants which can help reduce the use of animals for toxicity testing.
Citation
Borenfreund, Ellen, and James a. Puerner. “TOXICITY DETERMINED IN VITRO BY MORPhOLOGICaL aLTERaTIONS aND NEUTRaL RED aBSORPTION.” Toxicology Letters 24 (1985): 119–24.
