Effect of e-cigarette refill liquid on follicular development and estrogen secretion in rats.

Introduction:
This text is a research paper that examines the effect of e-cigarette refill liquid (e-liquid) on follicular development and estrogen secretion in rats and whether it is related to the hippo signaling pathway. The study aims to investigate the potential public health hazards of e-cigarettes and their impact on ovarian function.

Key Points:

* The study used ovaries from 21- and 35-day-old rats, which were divided into three groups: control, 0.05 mg, and 0.5 mg (e-liquids containing 0.5 mg and 5 mg of nicotine/kg).
* The ovaries were cultured for three hours in vitro and subjected to hE staining, immunohistochemistry, Western blotting, and ELISa to assess follicle morphology, expression of hippo signaling pathway factors, and estrogen concentrations.
* The results showed that e-liquid intervention decreased the percentage of normal follicles, decreased the expression of CYP19 and YaP, and decreased estrogen concentrations.
* Western blotting showed that CYP19, LaTS2, and YaP expression decreased, while PLaTS2 and PYaP expression increased after e-liquid intervention.
* The study suggests that e-liquids may impair rat ovarian follicle development and reduce estrogen secretion through the hippo signaling pathway.

Main Message:
The study highlights the potential public health hazards of e-cigarettes and their impact on ovarian function. The findings suggest that e-liquids may impair rat ovarian follicle development and reduce estrogen secretion through the hippo signaling pathway. Further research is needed to confirm these findings and investigate the potential impact of e-cigarettes on human ovarian function. as e-cigarette use continues to rise, particularly among young people, it is crucial to understand the potential risks associated with their use and take appropriate regulatory measures to protect public health.

Chen T, Wu M, Dong Y, et al. Effect of e-cigarette refill liquid on follicular development and estrogen secretion in rats. Tobacco induced diseases. 2022;20:36. doi:10.18332/tid/146958