Coresta In Vitro Toxicology Taskforce: The Rationale and Strategy For Conducting In Vitro Toxicology Testing of Tobacco Smoke

Introduction:
This text outlines the rationale and strategy for conducting in vitro toxicology testing of tobacco smoke, as recommended by the Coresta Scientific Committee. The text includes the recommended assays, their modifications for tobacco smoke testing, and the procedures for smoke preparation and data interpretation.

Key Points:

* The Coresta In Vitro Toxicology Taskforce recommends a test battery composed of three assays: ames Salmonella mutagenicity assay, mammalian cell assay for cytogenetics/mutation, and cytotoxicity assay conducted with an appropriate mammalian cell line.
* The ames Salmonella mutagenicity assay measures the ability of tobacco smoke to produce mutations in specific strains of Salmonella bacteria.
* The mammalian cell assay for cytogenetics/mutation measures structural changes and rearrangements in chromosomes resulting from DNa damage.
* The cytotoxicity assay conducted with an appropriate mammalian cell line measures the effect of tobacco smoke on cell viability and growth rates.
* Smoke preparation should be carried out according to ISO, FTC, or Coresta recommendations, and pads should be stored below -70 degrees Celsius if not used immediately.
* The ames test for tobacco smoke should include all tester strains, both with and without S9 metabolic activation.
* In vitro micronucleus assay results should be confirmed with replicate experiments if the control smoke condensate is negative or if the first experiment demonstrates lower or neutral activity of the test sample relative to the control sample.
* Neutral red cytotoxicity assay data should be confirmed in a replicate experiment, and exposure to the agent of interest should last at least one cell cycle.

Main Message:
The Coresta In Vitro Toxicology Taskforce recommends a test battery composed of three assays for conducting in vitro toxicology testing of tobacco smoke. These assays, when properly conducted, provide a scientifically sound foundation for assessing the in vitro toxicity of tobacco smoke. The text includes detailed procedures for smoke preparation and data interpretation, ensuring that the testing is conducted in a standardized and reliable manner. By following these recommendations, regulatory agencies can assess the toxicity of tobacco smoke and make informed decisions about its safety.

“Coresta In Vitro Toxicology Taskforce: The Rationale and Strategy For Conducting In Vitro Toxicology Testing of Tobacco Smoke.” CORESTa, 2004.