Summary
Introduction:
This document, CRM No. 62, details the CORESTa Recommended Method for the determination of nicotine in tobacco and tobacco products by gas chromatographic analysis. This summary will provide key points about the method's development, scope, and validation.
Key Points:
* The method was developed through an international collaborative study involving 37 laboratories and various tobacco sample types.
* The gas chromatographic procedure using MTBE as a solvent and the gas chromatographic procedure using n-hexane were evaluated and found to be suitable for nicotine determination.
* The method is applicable to ground tobacco, cigarette filler, ground cigar filler, smokeless tobacco products, and nicotine pouches, with a calibration range of 0.0005 mg/ml to 0.8 mg/ml using MTBE as a solvent and 0.0006 mg/ml to 0.96 mg/ml using n-hexane as a solvent.
* all reagents must be of analytical grade or better, and standards should be prepared in amber or light-protected glassware.
* The method has been validated for repeatability and reproducibility in accordance with ISO 5725-2:1994.
* The TTPa Sub-Group completed a collaborative study in 2021 to expand the scope of the method to include nicotine pouches, which do not contain tobacco leaf.
* Nicotine pouches were analyzed using the MTBE method, and the repeatability and reproducibility values were assessed in general accordance with ISO 5725-2:1994.
Main Message:
The CORESTa Recommended Method CRM No. 62 is a validated and reliable method for determining nicotine content in various tobacco products, including traditional and very low nicotine content tobacco and tobacco products, as well as nicotine pouches. This method is widely accepted and utilized by the tobacco industry and regulatory bodies for quality control, product development, and compliance purposes.
Citation
https://www.coresta.org/sites/default/files/technical_documents/main/CRM_62-December2021.pdf
