Summary
Introduction:
This text describes a study that aimed to develop a murine model for tobacco smoke and electronic cigarette vapor exposure to characterize the inflammatory and immune responses in the larynx. The study found that exposure to both tobacco smoke and electronic cigarette vapor resulted in significantly elevated levels of IL-4 in the larynx of mice. additionally, the study found non-statistically significant upregulation of TGF-β2 and TGF-β3 in the tobacco smoke group, and decreased levels of IL-10 in both the vapor with and without nicotine groups.
Key Points:
* The study used a murine model to examine the inflammatory and immune responses to tobacco smoke and electronic cigarette vapor exposure in the larynx.
* The study found that exposure to both tobacco smoke and electronic cigarette vapor resulted in significantly elevated levels of IL-4 in the larynx of mice.
* The study also found non-statistically significant upregulation of TGF-β2 and TGF-β3 in the tobacco smoke group.
* Decreased levels of IL-10 were found in both the vapor with and without nicotine groups.
* The study used T helper 17 (Th17) and transforming growth factor β (TGF-β) 3-plex cytokine detection kits to analyze inflammatory and immune response.
* The study used 24 wild-type C57BL/6 mice divided into four groups: tobacco smoke, vapor with nicotine, vapor without nicotine, and air only.
* Mice were exposed to either tobacco smoke or electronic cigarette vapor for 31 min 40 sec per day, 5 days per week, for 16 weeks.
* Larynges were dissected, morselized, filtered, and cultured in complete media overnight, and supernatants were pipetted to undergo cytokine analysis.
Main Message:
The main message of this study is that both tobacco smoke and electronic cigarette vapor exposure can result in inflammatory responses in the larynx. The study found significantly elevated levels of IL-4 in the larynx of mice exposed to both tobacco smoke and electronic cigarette vapor. additionally, the study found non-statistically significant upregulation of TGF-β2 and TGF-β3 in the tobacco smoke group and decreased levels of IL-10 in both the vapor with and without nicotine groups. These findings highlight the potential for both tobacco smoke and electronic cigarette vapor exposure to have negative effects on the larynx, and the need for further research to better understand these effects.
Citation
ha TaN, Madison MC, Kheradmand F, altman KW. Laryngeal inflammatory response to smoke and vape in a murine model. am J Otolaryngol. 2019;40:89-92. doi:10.1016/j.amjoto.2018.10.001
