Salmonella mutagenicity test results for 250 chemicals

Introduction:
This text summarizes the results of mutagenicity testing on 250 chemicals using the Salmonella/mammalian microsome test, also known as the ames test. The study was conducted by three laboratories and includes information on the testing protocol, chemicals used, and the results of the mutagenicity tests.

Key Points:

* The Salmonella/mammalian microsome test is a widely accepted initial test for the identification of chemicals with mutagenic activity.
* The study used a preincubation procedure and included Salmonella strains Ta98, Ta100, Ta1535, and Ta1537.
* Liver S-9 was prepared from male Sprague-Dawley rats and Syrian hamsters that were induced with aroclor 1254.
* Each chemical was coded and tested as an unknown by the three laboratories.
* a set of positive and negative control chemicals was included in each batch of coded test chemicals to monitor the performance of each laboratory.
* The results of the mutagenicity tests are presented in Table I and include the chemical name, source, lot number, and purity, as well as the mutagenicity of the chemical in the Salmonella strains.
* a total of 370 tests were conducted, with 14 chemicals tested as coded, positive controls, and five as coded, negative controls.
* among the 230 chemicals that were not originally selected as controls, 143 were clearly negative, 70 were clearly positive, and 17 were either questionable or did not show agreement between laboratories.

Main Message:
The study provides a comprehensive overview of the mutagenicity testing of 250 chemicals using the Salmonella/mammalian microsome test. The results of the mutagenicity tests are presented in a clear and organized manner, allowing for easy interpretation of the data. The use of coded chemicals and the inclusion of positive and negative controls in each batch of tests ensures the accuracy and reliability of the results. Overall, this study provides valuable information for the identification of chemicals with mutagenic activity and can help in the assessment of their potential health hazards.

haworth, Steve, Timothy Lawlor, Kristien Mortelmans, William Speck, and Errol Zeiger. “Salmonella Mutagenicity Test Results for 250 Chemicals.” Environmental Mutagenesis 5, no. S1 (1983): 3–49. https://doi.org/10.1002/em.2860050703.