Summary
Introduction:
This text reports the results of a study in which 47 chemical substances were tested for genotoxicity using the micronucleus test in mice. The study also includes data from the ames test and the chromosomal aberration test in vitro for some of the compounds. The study aimed to identify genotoxic compounds and evaluate their potential risk to human health.
Key points:
* The study tested 47 chemical substances, including food additives, insecticides, and industrial chemicals.
* The micronucleus test was performed in mice using a single ip injection, multiple treatments, or both.
* Five compounds were found to induce micronuclei after a single ip injection.
* Potassium bromate, sodium chlorite, and sodium dehydroacetate were positive in both the ames test and the chromosomal aberration test.
* Potassium bromate was also positive in the oral administration test.
* almost all the compounds that were negative with the single treatment were also negative in the multiple treatment tests.
* The study suggests that substances positive in vitro are not necessarily positive in vivo.
Main message:
The study highlights the importance of in vivo genotoxicity testing in addition to in vitro tests for the evaluation of chemical safety. The micronucleus test in mice was found to be a sensitive method for detecting genotoxic compounds. however, the study also shows that substances that are positive in vitro may not be genotoxic in vivo. Therefore, a combination of in vitro and in vivo tests is necessary to ensure the safety of chemical substances. The study also emphasizes the need for further studies to determine the mechanism of action of genotoxic compounds and their potential risks to human health.
Citation
hayashi, M., M. Kishi, T. Sofuni, and M. Ishidate. “Micronucleus Tests in Mice on 39 Food additives and Eight Miscellaneous Chemicals.” Food and Chemical Toxicology 26, no. 6 (January 1988): 487–500. https://doi.org/10.1016/0278-6915(88)90001-4.
