Mainstream smoke Constituents and In Vitro toxicity comparative analysis of 3R4F and 1R6F reference cigarettes

A new Kentucky reference cigarette, 1R6F, has been manufactured to replace the depleting 3R4F reference
cigarette.The3R4FKentuckyreferencecigaretteshavebeenwidelyusedasmonitororcomparatorcigarettesfor
mainstream smoke analysis and in vitro and in vivo toxicological data of cigarettes and novel tobacco products.
Both reference cigarettes were analyzed in the same laboratory during the same period of time with the goal of
performing a comparison of 3R4F and 1R6F. On the basis of the results obtained from aerosol chemistry and in
vitro assays, we consider that the 1R6F reference cigarette is a suitable replacement for the 3R4F reference
cigarette as a comparator/monitor cigarette. Its specific use as a comparator for novel tobacco products was
checked on the basis of a comparative test with the Tobacco Heating System 2.2 as an example.

Jaccard, G., Djoko, D. T., Korneliou, A., Stabbert, R., Belushkin, M., & Esposito, M. (2019). Mainstream smoke constituents and in vitro toxicity comparative analysis of 3R4F and 1R6F reference cigarettes. Toxicology reports, 6, 222–231. https://doi.org/10.1016/j.toxrep.2019.02.009