This document discusses the history and development of analytic smoking machines used to generate and collect tobacco smoke for toxicology testing. The machines have evolved since their introduction in the 1930s, with varying sophistication, puffing protocols, and collection approaches. Despite attempts to standardize smoke extraction generation over the past 80 years, there is ongoing debate about the most appropriate methods and their relevance to what smokers are truly exposed to. The document highlights the importance of understanding the experimental designs, toxicologic endpoints, and their relevance to human disease risk when collecting and testing tobacco smoke.
Citation
Johnson, Michael D., Jodi Schilz, Mirjana V. Djordjevic, Jerry R. Rice, and Peter G. Shields. “Evaluation of In Vitro assays for assessing the Toxicity of Cigarette Smoke and Smokeless Tobacco.” Cancer Epidemiology, Biomarkers & Prevention 18, no. 12 (December 1, 2009): 3263–3304. https://doi.org/10.1158/1055-9965.EPI-09-0965.
Johnson, Michael D., Jodi Schilz, Mirjana V. Djordjevic, Jerry R. Rice, and Peter G. Shields. “Evaluation of In Vitro assays for assessing the Toxicity of Cigarette Smoke and Smokeless Tobacco.” Cancer Epidemiology, Biomarkers & Prevention 18, no. 12 (December 1, 2009): 3263–3304. https://doi.org/10.1158/1055-9965.EPI-09-0965.
