association of 1 Vaping Session With Cellular Oxidative Stress in Otherwise healthy Young People With No history of Smoking or Vaping: a Randomized Clinical Crossover Trial.

Introduction:
This text discusses a clinical study examining the association between a single session of electronic cigarette (ECIG) vaping and cellular oxidative stress (COS) in immune cells of young people who do not smoke or vape. The study also compares the effects of ECIG use to tobacco cigarette (TCIG) smoking in young people. The study's key points highlight the significant increase in COS in immune cells following a single ECIG vaping session and the lack of such increase in individuals who currently use TCIGs or ECIGs. The main message of the text underscores the potential health risks of ECIG use, even among occasional users, and emphasizes the need for further research to better understand the long-term health impacts of ECIG use.

Key Points:

* The study is a randomized clinical crossover trial examining the association between ECIG vaping and COS in immune cells of young people who do not smoke or vape.
* The study compares the effects of ECIG use to TCIG smoking in young people and finds that even a single ECIG vaping session increases COS in immune cells, while TCIG smoking and ECIG use in current users do not show such an increase.
* The study uses peripheral blood mononuclear cells and flow cytometry to determine COS in immune cells, with CellROX Green and CellROX Deep Red used for total and cytoplasmic reactive oxygen species content, respectively.
* The study measures COS in immune cell subtypes, including CD45+CD15+CD16+CD14−hi-SSC neutrophils, CD45+CD142+CD16− classic, CD45+CD142+CD16+ intermediate, CD45+CD14dimCD16+ nonclassic monocytes, CD45+CD3+CD4+ T cells, CD45+CD3+CD8+ T cells, CD45+CD3−CD56+CD16+ natural killer (NK) cells, and CD45+CD19+ B cells.
* The study finds that a single ECIG vaping session increases the percentage of CD142+CD16+ and CD14dimCD16+ monocytes, T cells, and natural killer cells that are positive for CellROX Green.
* The study's limitations include a small sample size, and strengths include the detection of COS in immune cells within the same person preintervention and postintervention.
* The study has implications for young people who have pivoted from TCIG smoking to ECIG vaping, as even occasional ECIG use can induce changes in oxidative stress levels.

Main Message:
The main message of the text is the potential health risks associated with ECIG use, even among occasional users. The study's findings suggest that a single ECIG vaping session can induce changes in oxidative stress levels in immune cells, which could lead to significant future pathologies, including cardiovascular, pulmonary, and neurological diseases. The text emphasizes the need for further research to better understand the long-term health impacts of ECIG use and highlights the importance of informing young people about the potential risks associated with ECIG use.

Kelesidis T, Tran E, Nguyen R, Zhang Y, Sosa G, Middlekauff hR. association of 1 Vaping Session With Cellular Oxidative Stress in Otherwise healthy Young People With No history of Smoking or Vaping: a Randomized Clinical Crossover Trial. JaMa pediatrics. 2021;175(11):1174-1176. doi:10.1001/jamapediatrics.2021.2351