E-cigarette aerosol induced cytotoxicity, DNa damages and late apoptosis in dynamically exposed a549 cells.

Introduction:
This text discusses a study examining the acute toxicological impacts of electronic cigarette (e-cigarette) usage through a novel dynamic exposure methodology. The methodology was used to test various e-cigarette generated aerosols in a549 cell cultures. The study identified numerous compounds in e-cigarette chemical analysis, including nicotine in advertised nicotine-free e-liquids. E-liquids displayed cytotoxic impacts in a549 cells, and dynamic exposure to e-liquids aerosols showed cytotoxic and genotoxic potentials. Late apoptosis was found to be the main mechanism of programmed cell death.

Key Points:

* The study utilized a novel dynamic exposure methodology to test e-cigarette generated aerosols in a549 cell cultures.
* E-cigarette chemical analysis identified numerous compounds, including nicotine in advertised nicotine-free e-liquids.
* E-liquids displayed cytotoxic impacts in a549 cells, and dynamic exposure to e-liquids aerosols showed cytotoxic and genotoxic potentials.
* Late apoptosis was found to be the main mechanism of programmed cell death.

Main Message:
The study highlights the significant risks posed by e-cigarette usage, with acute aerosol exposure inducing cytotoxic, genotoxic, and apoptotic induction in exposed cells. The proposed methodology proved to be a useful tool for future screening of e-liquids generated aerosols toxicity. however, further research is needed to establish the chronic toxicity resulting from long-term e-cigarette consumption. The findings underscore the importance of continuing to study the health impacts of e-cigarette usage and the need for effective regulation to protect public health.

Khalil C, Chahine JB, haykal T, al hageh C, Rizk S, Khnayzer RS. E-cigarette aerosol induced cytotoxicity, DNa damages and late apoptosis in dynamically exposed a549 cells. Chemosphere. 2021;263:127874. doi:10.1016/j.chemosphere.2020.127874