Transgenic chinese hamster V79 cell lines which exhibit variable levels of gpt mutagenesis

Introduction:
This text presents a study on the use of transgenic Chinese hamster V79 cells containing the Escherichia coli gpt gene, which confers resistance to the antibiotic thioguanine, to study mutagenesis. The study establishes several unique gpt+ transgenic V79 cell lines and characterizes their spontaneous gpt mutability. The mutagenic responsiveness of the gpt locus is compared with that of the endogenous hprt in wild-type V79 cells.

Key Points:

* The study established several unique gpt+ transgenic V79 cell lines with different integration sites and spontaneous mutability.
* The g12 transgenic cell line was found to have a relatively low frequency of spontaneous mutations and was more responsive to certain mutagens, including X-rays, compared to wild-type V79 cells.
* The integration site of the gpt target and the presence of multilocus deletions may affect its mutability.
* The transgenic g12 cells can detect mutagenesis by agents that exhibit limited hprt mutagenesis, making them useful for studying clastogens and mutagens that are difficult to detect in other mammalian mutation systems.
* The study also highlights the importance of understanding the factors that influence the mutability of transgenes to improve the accuracy of mutagenesis studies.

Main Message:
The study demonstrates the utility of transgenic Chinese hamster V79 cells containing the Escherichia coli gpt gene as a tool for studying mutagenesis. The study's findings suggest that the integration site and the presence of multilocus deletions can affect the mutability of transgenes. Therefore, understanding these factors is crucial for improving the accuracy of mutagenesis studies and identifying mutagens that may be difficult to detect in other mammalian mutation systems. Overall, the study underscores the importance of using appropriate experimental models and taking into account the various factors that can influence mutagenesis.

Klein, Catherine B., Toby G. Rossman, and T. R. Skopek. “Transgenic Chinese hamster V79 Cell Lines Which Exhibit Variable Levels of Gpt Mutagenesis.” Environmental and Molecular Mutagenesis 16, no. 1 (January 1990): 1–12. https://doi.org/10.1002/em.2850160102.