Nose-Only Exposure to Cherry- and Tobacco-Flavored E-Cigarettes Induced Lung Inflammation in Mice in a Sex-Dependent Manner.

Introduction:
This text discusses a study investigating the immune-inflammatory effects of exposure to flavored e-cigarettes in mice. The study quantified the concentration of specific flavoring chemicals in e-liquids and determined their inhalation effects. The research also explored the sex-specific effects of e-cigarette exposure on matrix metalloprotease protein levels in lung homogenate.

Key Points:

* The study exposed mice to air, PG/VG, and five distinct e-liquid flavors for three days.
* Researchers measured pro-inflammatory cytokines/chemokines in bronchoalveolar lavage fluid (BaLF) and lung homogenate.
* Male mice exposed to Smooth & Mild Tobacco showed a significant increase in neutrophil count in BaLF.
* Female mice exposed to PG/VG and Cherry had significantly increased KC and IL-6 levels in lung homogenate.
* Exposure to PG/VG, apple, Cherry, and Wintergreen resulted in a significant increase in MMP2 protein levels in female mice.
* Male mice exposed to apple displayed a significant decrease in MMP9 protein levels compared to air controls.
* Exposure to PG/VG, apple, Cherry, and Wintergreen resulted in a significant increase in MMP2 protein levels in male mice.

Main Message:
The study suggests that flavored e-cigarettes can have both pro-inflammatory and anti-inflammatory effects, depending on the specific flavor and sex of the mice. The research emphasizes the importance of further investigating the sex-specific effects of e-cigarette exposure to better understand their potential health risks. The findings also highlight the need for regulation of flavoring chemicals in e-cigarettes to ensure user safety.

Lamb T, Muthumalage T, Meehan-atrash J, Rahman I. Nose-Only Exposure to Cherry- and Tobacco-Flavored E-Cigarettes Induced Lung Inflammation in Mice in a Sex-Dependent Manner. Toxics. 2022;10(8). doi:10.3390/toxics10080471