Summary
Introduction:
This text discusses a study that investigated the formation of potentially toxic chlorinated compounds in e-cigarette aerosols when using sucralose-sweetened e-liquids. The study also examines the cytotoxicity of the aerosol condensates.
Key points:
* The study used e-liquids with propylene glycol and glycerol as the base, and added sucralose in different concentrations.
* The e-liquids were vaporized using a standardized e-cigarette at different temperatures and puff numbers.
* The vaporized e-liquid aerosol was trapped and condensed using liquid nitrogen.
* The condensates were analyzed using gas chromatography (GC-FID and GC-MS) to detect and quantify chlorinated compounds.
* The cytotoxicity of the condensates was tested on human umbilical vein endothelial cells (hUVEC/Tert2) using the resazurin assay.
* The study found significant amounts of chlorinated compounds in the condensates, including chloroacetaldehyde, 1-chloro-2-propanol, 2-chloro-1-propanol, 1,3-dichloro-2-propanol, 2,3-dichloro-1-propanol, and 3-chloropropane-1,2-diol.
* The cellular metabolic activity of hUVEC/Tert2 cells decreased significantly after treatment with e-liquid aerosol condensate.
Main message:
The study suggests that the use of sucralose-sweetened e-liquids in e-cigarettes can lead to the formation of potentially toxic chlorinated compounds in the aerosol. The results also demonstrate that these condensates have cytotoxic effects on hUVEC/Tert2 cells. Therefore, the study advises against the use of sucralose as a sweetener in e-liquids. This research is important for tobacco regulatory science and toxicology evaluations of new tobacco products, such as electronic nicotine delivery systems (ENDS).
Citation
Moser D, Leitner P, Filipek Pa, et al. quantification and cytotoxicity of degradation products (chloropropanols) in sucralose containing e-liquids with propylene glycol and glycerol as base. Toxicology and applied pharmacology. 2021;430:115727. doi:10.1016/j.taap.2021.115727
