Carbonyl Profiles of Electronic Nicotine Delivery System (ENDS) aerosols Reflect Both the Chemical Composition and the Numbers of E-Liquid Ingredients-Focus on the In Vitro Toxicity of Strawberry and Vanilla Flavors.

Introduction:
This text provides an in-depth analysis of a study examining the chemical profiles and in vitro toxicity of electronic nicotine delivery system (ENDS) aerosols. The study focuses on the impact of various factors, such as humectant ratios, flavors, and nicotine content, on the aerosol's composition and potential health effects.

Key Points:

1. The study determined the chemical profiles of ENDS aerosols containing three humectant ratios, three flavors, and two nicotine concentrations.
2. Third-generation ENDS aerosols composed solely of humectants can contain elevated levels of acetaldehyde and formaldehyde.
3. The addition of flavoring chemicals to e-liquids can either increase or decrease the formation of aldehydes during heating and aerosolization.
4. Carbonyl levels emitted from fourth-generation ENDS are much lower than those emitted from third-generation devices.
5. an acute 1-hour exposure of BEaS-2B cells to vanilla-flavored e-cig aerosol at the aLI was not cytotoxic but significantly dysregulated the expression of several genes involved in biotransformation, inflammation, and oxidative stress processes.
6. The strawberry-flavored e-cig aerosol induced oxidative stress responses, while the vanilla-flavored e-cig aerosol mainly activated pro-inflammatory pathways.
7. The findings suggest that flavoring chemicals in e-liquids could be regulated by governmental agencies to better protect ENDS users of all ages.

Main Message:
The study highlights the importance of considering various factors, such as humectant ratios, flavors, and nicotine content, when evaluating the potential health risks associated with ENDS aerosol exposure. The findings suggest that certain flavoring chemicals, like vanillin, could have adverse effects following chronic exposures to vanilla-flavored e-cig aerosols. additionally, the study emphasizes the need for further research on the pulmonary effects of heated and aerosolized flavoring chemicals to inform regulatory decisions and protect ENDS users, particularly youth and young adults, from potential health hazards.

Noël a, Ghosh a. Carbonyl Profiles of Electronic Nicotine Delivery System (ENDS) aerosols Reflect Both the Chemical Composition and the Numbers of E-Liquid Ingredients-Focus on the In Vitro Toxicity of Strawberry and Vanilla Flavors. International journal of environmental research and public health. 2022;19(24). doi:10.3390/ijerph192416774