Effect of e-cigarettes on nasal epithelial cell growth, Ki67 expression, and pro-inflammatory cytokine secretion.

Introduction:
This text provides an analysis of a scientific study examining the effects of e-cigarettes on nasal epithelial cells and tissue. The study compares e-cigarette aerosol to standard cigarette smoke and assesses their impact on cell viability, lactate dehydrogenase (LDh) activity, tissue structure, and pro-inflammatory cytokine secretion.

Key Points:

* The study used human primary nasal epithelial cells and engineered 3D nasal mucosa tissues.
* Exposure to e-cigarette aerosol resulted in larger cell size, increased LDh activity, and structural deregulation in nasal tissues.
* Tissues exposed to e-cigarette aerosol displayed fewer Ki67-positive cells and a reduced proliferation rate.
* Cytokine measurements showed high levels of IL-6, IL-8, TNFα, and MCP-1, indicating that e-cigarettes activated pro-inflammatory cytokine responses.
* The findings suggest that e-cigarettes could be a threat to nasal tissues and may impair the innate immune function of nasal epithelial cells.
* The study also discusses the potential risks of e-cigarettes to oral health and their association with severe pulmonary diseases.

Main Message:
The study highlights the potential harmful effects of e-cigarettes on nasal epithelial cells and tissue. The findings suggest that e-cigarette aerosol can cause structural and functional changes in nasal tissue, leading to inflammation and impaired immune function. as such, it is crucial to consider these risks when evaluating the overall safety and health impact of e-cigarettes.

Rouabhia M, Piché M, Corriveau MN, Chakir J. Effect of e-cigarettes on nasal epithelial cell growth, Ki67 expression, and pro-inflammatory cytokine secretion. american journal of otolaryngology. 2020;41(6):102686. doi:10.1016/j.amjoto.2020.102686