The study used the ames test, a well-established bacterial reverse mutation assay, to evaluate the mutagenic activity of cigarette smoke condensate with and without the S9 fraction, which is used to simulate the metabolic activation of chemicals in the human body. Positive control chemicals were used to ensure the assay was working efficiently. The study found that cigarette smoke condensate induced a mutagenic response in the ames test, and the response was enhanced in the presence of the S9 fraction. however, the specific results and details of the experiments are not provided in the text, and the document only serves as an introduction to the study. Further information can be found in the supplementary data and transparency document, which are linked in the article. It is important to note that the research was solely funded by Philip Morris, and all authors are (or were) employees of PMI R&D or worked for PMI R&D under contractual agreements.
Citation
Schaller, Jean-Pierre, Daniela Keller, Laurent Poget, Pascal Pratte, Etienne Kaelin, Damian Mchugh, Gianluca Cudazzo, et al. “Evaluation of the Tobacco heating System 2.2. Part 2: Chemical Composition, Genotoxicity, Cytotoxicity, and Physical Properties of the aerosol.” Regulatory Toxicology and Pharmacology 81 (November 2016): S27–47. https://doi.org/10.1016/j.yrtph.2016.10.001.
Schaller, Jean-Pierre, Daniela Keller, Laurent Poget, Pascal Pratte, Etienne Kaelin, Damian Mchugh, Gianluca Cudazzo, et al. “Evaluation of the Tobacco heating System 2.2. Part 2: Chemical Composition, Genotoxicity, Cytotoxicity, and Physical Properties of the aerosol.” Regulatory Toxicology and Pharmacology 81 (November 2016): S27–47. https://doi.org/10.1016/j.yrtph.2016.10.001.
