Toxicological assessment of Flavor Ingredients in E-Vapor Products.

Introduction:
This text describes a proposed approach for assessing the toxicology of flavor ingredients in electronic vapor products (EVPs). The authors propose a method that groups structurally similar flavor ingredients and selects representative ingredients from each group for toxicological testing. This method aims to reduce the number of tests required and minimize the use of animal subjects.

Key Points:

* The proposed approach involves grouping flavor ingredients based on structural, toxicological, and metabolic properties.
* Flavor ingredients are assigned to one of 38 groups, which are further divided into subgroups based on the European Commission (EC) Regulation No 1565/2000.
* a representative flavor ingredient (FGR) is selected from each group based on available experimental and predicted toxicological data.
* The FGRs are then used to create a representative flavor mixture (RFM) for toxicological testing.
* The approach includes in vitro and in vivo testing of the RFMs, as well as in silico predictions of toxicological properties.
* The authors present a case study of the approach, using 245 commonly used flavor ingredients.
* The approach has the potential to reduce the time and resources required for toxicological testing, while also minimizing the use of animal subjects.

Main Message:
The proposed approach for assessing the toxicology of flavor ingredients in EVPs has the potential to significantly reduce the number of tests required and minimize the use of animal subjects. By grouping structurally similar ingredients and selecting representative ingredients for testing, the authors aim to improve the efficiency and effectiveness of toxicological testing for EVP flavor ingredients. The case study presented in the text demonstrates the feasibility of this approach and highlights its potential benefits.

Sciuscio D, Calvino-Martin F, Kumar a, et al. Toxicological assessment of Flavor Ingredients in E-Vapor Products. Frontiers in toxicology. 2022;4:878976. doi:10.3389/ftox.2022.878976