Summary
Introduction:
This article reports a genotoxicity evaluation of tobacco and nicotine delivery products, specifically using the in vitro micronucleus assay. The study compares various test matrices from tobacco and nicotine delivery products to a 3R4F Kentucky reference cigarette. The research was conducted using two Chinese hamster cell lines and a human lymphoblastoid cell line in accordance with established guidelines.
Key points:
* The in vitro micronucleus assay was performed on three cell lines with 3R4F TPM and other tobacco and nicotine product test matrices.
* Cells exposed to 3R4F TPM showed varied responses, with the most robust response in V79 cells.
* The extended exposure/recovery period increased assay sensitivity for ChO and V79 cell lines, but the effect was less clear for TK6 cells.
* Negative responses were observed for all products except 3R4F across all treatment conditions in V79 cells.
* The most potent response to cigarette smoke was following extended treatment with recovery, suggesting this may be a more appropriate treatment for the future assessment of tobacco and nicotine product test matrices.
Main message:
The study highlights the potential of the in vitro micronucleus assay to evaluate the genotoxicity of tobacco and nicotine delivery products. The findings suggest that the extended exposure/recovery period may enhance assay sensitivity. Furthermore, the results indicate that the test matrices from the tobacco and nicotine delivery products, except for 3R4F, did not show genotoxic effects in V79 cells. This research supports the use of the in vitro micronucleus assay in the initial screening of the toxicological potential of NGPs.
Citation
Thorne D, Leverette R, Breheny D, et al. Genotoxicity evaluation of tobacco and nicotine delivery products: Part Two. In vitro micronucleus assay. Food and chemical toxicology : an international journal published for the British Industrial Biological Research association. 2019;132:110546. doi:10.1016/j.fct.2019.05.054
