Comparative analysis of the impact of e-cigarette vapor and cigarette smoke on human gingival fibroblasts.

Introduction:
This text provides an analysis of the impact of electronic cigarette (eCV) vapor and traditional cigarette smoke (CS) on human gingival fibroblasts (hGF) in terms of proliferation, metabolic activity, cell death, and reactive oxygen species (ROS) formation. The study aims to compare the effects of eCV and CS on hGF using an exposure chamber.

Key Points:

* The study used an exposure chamber to treat hGF with eCV, CS, or ambient air for 15 minutes and monitored the cells' proliferation, metabolic activity, caspase 3/7 activation, apoptosis and necrosis, and ROS formation.
* CS-exposed cells showed significantly decreased cell numbers compared to the control cells after 24 hours, while eCV-exposed cells showed a significantly higher metabolic activity at multiple time points.
* No significant differences were found in caspase 3/7 activation or the percentage of apoptotic, necrotic, or dead cells among the groups.
* ROS formation was significantly increased in CS-exposed cells compared to controls and eCV-exposed cells at multiple time points.
* The study indicates that CS has a greater impact on proliferation and ROS formation than eCV, while eCV mainly affects cellular metabolic activity.
* This is the first study to evaluate the effects of eCV on hGF using an exposition chamber.
* The study's findings suggest that eCV does not seem to be as harmful as traditional CS for hGF, but further studies are needed to understand the potentially harmful effects of e-cigarettes on the oral mucosa.

Main Message:
The study provides valuable insights into the impact of eCV and CS on hGF, highlighting the potential benefits of eCV over traditional CS. The findings can contribute to the development of regulations and policies regarding the use of eCV and the promotion of public health.

Vermehren MF, Wiesmann N, Deschner J, Brieger J, al-Nawas B, Kämmerer PW. Comparative analysis of the impact of e-cigarette vapor and cigarette smoke on human gingival fibroblasts. Toxicology in vitro : an international journal published in association with BIBRa. 2020;69:105005. doi:10.1016/j.tiv.2020.105005