In Vitro Toxicological Investigation and Risk assessment of E-Cigarette aerosols Based on a Novel Solvent-Free Extraction Method.

Introduction:
This text provides an in-depth analysis of the toxicological investigation and risk assessment of e-cigarette aerosols based on a novel solvent-free extraction method. It discusses the development of a high-speed centrifugal method for extracting e-cigarette aerosol collected mass (aCM) and the preparation of aCM samples of 26 representative e-cigarettes. The text also highlights the toxicological evaluation of the samples and the comparison of the toxicity difference among e-cigarettes using nicotine equivalent and aCM.

Key Points:

* a high-speed centrifugal method was developed to extract e-cigarette aerosol collected mass (aCM) with an average extraction efficiency of over 85%.
* The toxicological evaluation showed that e-cigarettes can induce the risk of DNa damage, mitochondrial depolarization, and c-Jun-related signal disturbances.
* Most e-cigarettes significantly caused disturbance of oxidative stress balance.
* The toxicity difference among e-cigarettes using nicotine equivalent was significantly lower than that of aCM.
* The study provides a comprehensive in vitro toxicity risk profile of e-cigarette aerosols.

Main Message:
The study highlights the importance of a simple and efficient extraction method for toxicological evaluations of e-cigarette aerosols. The novel solvent-free extraction method developed in this study provides a reliable and efficient way to extract aCM samples, which can be used for comprehensive in vitro toxicity evaluations. The toxicological evaluation showed that e-cigarettes can cause various health risks, including DNa damage, mitochondrial depolarization, and c-Jun-related signal disturbances. The study also emphasizes that the toxicity difference among e-cigarettes using nicotine equivalent is significantly lower than that of aCM, indicating the need for further research to understand the toxicity of e-cigarette aerosols fully. Overall, the study provides valuable insights into the toxicological profile of e-cigarette aerosols and highlights the need for continued research and regulation to ensure public health safety.

Wang h, han S, Chen h, et al. In Vitro Toxicological Investigation and Risk assessment of E-Cigarette aerosols Based on a Novel Solvent-Free Extraction Method. aCS omega. 2022;7(51):48403-48415. doi:10.1021/acsomega.2c06663