Cytotoxic and genotoxic effects of e-liquids and their potential associations with nicotine, menthol and phthalate esters.

DNa damage and chromosome breakage (indicators of genotoxicity) and cell viability (an indicator of cytotoxicity) in human lymphoblastoid TK6 and Chinese hamster ovary (ChO) cells treated with 33 e-liquids using in vitro single cell gel (comet), micronucleus (MN), and trypan blue assays, respectively. all liquids induced DaN damage, however a sub-group of 20 liquids led to ≤75% cell viability.

al-Saleh I, Elkhatib R, al-Rajoudi T, et al. Cytotoxic and genotoxic effects of e-liquids and their potential associations with nicotine, menthol and phthalate esters. Chemosphere. 2020;249:126153. doi:10.1016/j.chemosphere.2020.126153