The combination of two novel tobacco blends and filter technologies to reduce the in vitro genotoxicity and cytotoxicity of prototype cigarettes

Introduction:
This text discusses a study that aimed to assess the genotoxicity and cytotoxicity of a novel experimental cigarette, which incorporates various tobacco blend and filter technologies, using a battery of in vitro assays. The study also compared the results to control cigarettes. The key points and main message of the text are summarized below.

Key Points:

* The experimental cigarette contained 50% blend treated tobacco (BTT), 15% tobacco sheet substitute (TSS), polymer-derived activated charcoal, CR20L, and split tipping (ST) technologies.
* Control cigarettes included a commercial control (CC) and a baseline control (BC) cigarette, as well as 3R4F and M4a reference cigarettes.
* PM was generated under ISO and hCI smoking regimes and tested in the ames test, MLa, MNvit, and NRU.
* The experimental cigarette showed reduced mutagenicity in the ames test in Ta98 and Ta100 strains with S9, but not Ta1537.
* The experimental cigarette was less genotoxic than the commercial control in the MLa 3h treatment, but not in the 24h treatment.
* The experimental cigarette was less genotoxic than the baseline control in the MNvit, but not in the NRU.
* The study suggests that the experimental cigarette may reduce the genotoxicity and cytotoxicity of cigarette smoke without introducing new hazards.

Main Message:
The study provides evidence that the experimental cigarette, which incorporates various tobacco blend and filter technologies, may reduce genotoxicity and cytotoxicity without introducing new hazards. The results suggest that these technologies could be used to develop cigarettes with reduced toxicant emissions, which may help reduce the health risks associated with smoking. however, further studies are needed to confirm these findings and evaluate the impact of these technologies on smoking behavior and health outcomes.

Crooks, Ian, Ken Scott, annette Dalrymple, Debbie Dillon, and Clive Meredith. “The Combination of Two Novel Tobacco Blends and Filter Technologies to Reduce the in Vitro Genotoxicity and Cytotoxicity of Prototype Cigarettes.” Regulatory Toxicology and Pharmacology 71, no. 3 (april 2015): 507–14. https://doi.org/10.1016/j.yrtph.2015.01.001.