Refill liquids for electronic cigarettes display peculiar toxicity on human endothelial cells.

Introduction:
This text is a summary of a scientific study that investigates the cytotoxicity of different refill liquids for electronic cigarettes (e-cigarettes) on human endothelial cells. The study aims to assess the toxicity of these liquids using two different in vitro approaches: the resazurin and lactate dehydrogenase (LDh) release assays. The study also compares the cytotoxicity of e-cigarette refill liquids to that of traditional cigarette condensates.

Key points:

* The study used six different refill liquids for e-cigarettes and three traditional cigarette condensates.
* The cytotoxicity of the liquids was evaluated using two different in vitro assays: resazurin and LDh release assays.
* The results showed that different refill liquids have different levels of cytotoxicity, although they were always lower than that observed for traditional cigarette condensates.
* The study found that two out of the six refill liquids did not show any cytotoxicity at all, whereas the other four caused a significant decrease in cell viability when used at high concentrations.
* The traditional cigarette condensates were significantly more toxic than the e-cigarette refill liquids.
* The study suggests that accurate evaluations should be provided for refill liquids, with a focus on correlating their toxicity to their chemical composition.
* The main message of the study is that further studies are needed to obtain useful information for the agencies involved in the regulation of refill liquid components.

Main message:
The study highlights the importance of accurate evaluations of the cytotoxicity of e-cigarette refill liquids. The results suggest that different refill liquids have different levels of toxicity, and some may not be harmful at all. however, the study also shows that traditional cigarette condensates are significantly more toxic than e-cigarette refill liquids. Therefore, it is crucial to provide accurate evaluations of the toxicity of refill liquids and to correlate their toxicity with their chemical composition. This information is essential for the agencies involved in the regulation of refill liquid components to make informed decisions and ensure public safety.

De Martin, S., Gabbia, D., Bogialli, S., Biasioli, F., Boschetti, A., Gstir, R., Rainer, D., & Cappellin, L. (2021). Refill liquids for electronic cigarettes display peculiar toxicity on human endothelial cells. Toxicology reports, 8, 456–462. https://doi.org/10.1016/j.toxrep.2021.02.021