Chronic E-Cigarette Exposure Alters the Human Bronchial Epithelial Proteome

Introduction: This text provides an in-depth analysis of the effects of chronic e-cigarette use on the lung, specifically focusing on changes in the proteome of airway epithelia. The study design includes recruiting healthy smokers, e-cigarette users (vapers), and nonsmoking control subjects and performing research bronchoscopies to obtain brush biopsy and lavage samples. The researchers also vaped human bronchial epithelial cultures and mice to test whether they could replicate their in vivo observations.

Key Points:

* Chronic e-cigarette use alters approximately 200 proteins in airway epithelia.
* In vitro and in vivo studies confirm these changes.
* Vaping causes erythematous and irritable airway mucosa.
* Proteomic alterations in vapers are distinct from tobacco exposure.
* PG/VG reduces membrane fluidity and impairs protein diffusion.
* E-liquids rapidly enter cells, affecting cellular function.
* Altered mucin expression may be useful as a biomarker of exposure and harm.
* Further studies are required to determine the full extent of vaping on the lung.

Main Message: The text highlights the potential harm of chronic e-cigarette use on the lung, with proteomic alterations suggesting that vaping is not harmless. The study emphasizes the need for further research to understand the long-term effects of vaping on lung health. Additionally, the findings suggest that e-cigarette regulations should consider the potential risks associated with PG/VG, a common base in most e-liquids. Overall, the text underlines the importance of raising awareness about the potential dangers of e-cigarette use and the need for rigorous regulation and oversight.

Ghosh, Arunava, Raymond C. Coakley, Teresa Mascenik, Temperance R. Rowell, Eric S. Davis, Keith Rogers, Megan J. Webster, et al. “Chronic E-Cigarette Exposure Alters the Human Bronchial Epithelial Proteome.” American Journal of Respiratory and Critical Care Medicine 198, no. 1 (July 1, 2018): 67–76. https://doi.org/10.1164/rccm.201710-2033OC.